Epilepsy

Creative Biogene is a leading biotechnology company offering the best Pseudomonas genome editing services. With years of experience and expertise in microbial genome editing, our talented scientists will work closely with you to provide any help in Pseudomonas genome editing services.

Pseudomonas species, a large class of gram-negative bacteria, exhibit significant biomedical, ecological, and industrial importance. Pseudomonas, a major human pathogen, is able to cause severe infections in vulnerable patients hospitalized with cystic fibrosis, burns, acquired immunodeficiency syndrome, or cancer. Extensive research has been focused to dissect the molecular basis of the infection mechanisms and to develop novel therapeutic means against Pseudomonas infections. The genetic manipulation in Pseudomonas is extremely urgent.

Our Pseudomonas genome editing services are based on CRISPR/Cas9 technology and homologous recombination technique. The state-of-art Pseudomonas editing system helps you successfully achieve gene knockout, gene insertion and point mutation for either research or industrial purposes.

Red/ET Recombination permits the engineering of DNA in LAB using homologous recombination mediated by phage protein pairs, either RecE/RecT or Reda/Redb. The central step in Red/ET recombination is the crossover step between a targeting construct containing homology arms and the target which can be a gene locus on the LAB chromosome by designing a homologous fusion fragment of the target gene, it is cloned into a suicide vector, and the suicide vector is transformed into the target Pseudomonas. An insertion mutant is selected by antibiotic screening. Under the second round of reverse selection pressure, only the mutation that contain second homologous recombination and the loss of the suicide plasmid can survive. By PCR screening and sequencing, we can obtain the mutant of the strain.

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    Leslie Perry Grayson